• Product NameNCK2 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S01-3C6
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human Nck beta
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    GRB4; NCKbeta
• Accession No. Swiss-Prot:O43639
  GeneID:8440
• UniprotO43639
• Gene ID8440
• Calculated MW Calculated MW: 43 kDa; Observed MW: 43 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000-1/5000; ICC/IF: 1/20-1/50

Swiss-Prot Acc.O43639.Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.

• Western blot detection of Nck beta in K562,Rat Brain,C6,3T3,Hela cell lysates using Nck beta Rabbit mAb(1:1000 diluted).Predicted band size:43kDa.Observed band size:43kDa.
• Immunofluorescence of Nck beta(green) in Hela cells using Nck beta Rabbit mAb at dilution 1/200, and DAPI(blue)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting