• Product NameNF kappa B p100 Rabbit mAb
• Clone No.S04-4D6
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB;ICC/IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant protein of human NFkB p100
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2; p49/p100
• Accession No. Swiss-Prot:Q00653
  GeneID:4791
• UniprotQ00653
• Gene ID4791
• Calculated MW Calculated Mol Weight 97 kDa Observed Mol Weight 120 kDa
• Concentration 0.3 mg/ml
• Formulation Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB 1:1000-1:2000
  ICC/IF 1:50-1:200

NFkB-p100 a transcription factor of the nuclear factor-kappaB ( NFkB) group. Precursor of the p52 subunit of the nuclear factor NF-kappa-B, which binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions.

• Immunofluorescence of NFkB p100(green) in Hela cells using NFkB p100 Rabbit mAb at dilution 1/200, and DAPI(blue)
• Western blot analysis of NF-κB p100 expression in (1) Jurkat cell lysate; (2) Mouse heart lysate.
• All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting