• Product NamePrion Protein Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S03-2E2
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human Prion protein PrP
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    CJD; GSS; PrP; ASCR; KURU; PRIP; PrPc; CD230; AltPrP; p27-30; PrP27-30; PrP33-35C
• Accession No. Swiss-Prot:P04156
  GeneID:5621
• UniprotP04156
• Gene ID5621
• Calculated MW Calculated MW: 28 kDa; Observed MW: 28 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000-1/5000; IHC: 1/20;

Swiss-Prot Acc.P04156.Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) . Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu2+ or ZN2+ for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains .

• Western blot detection of Prion protein in Rat Brain and Mouse Brain lysates using Prion Protein Rabbit mAb (1:1000 diluted).Predicted band size:28kDa.Observed band size:28kDa.
• Immunohistochemistry of Prion protein in paraffin-embedded Human breast cancer tissue using Prion Protein Rabbit mAb at dilution 1/20

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting