• Product NameSF3B3 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S07-2K8
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human SF3B3
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    RSE1; SAP130; SF3b130; STAF130
• Accession No. Swiss-Prot:Q15393
  GeneID:23450
• UniprotQ15393
• Gene ID23450
• Calculated MW Calculated MW: 136 kDa; Observed MW: 136 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000; ICC/IF: 1/50;

Swiss-Prot Acc.Q15393.Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome (PubMed:10490618, PubMed:10882114, PubMed:27720643, PubMed:28781166). SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937). May also be involved in the assembly of the 'E' complex (PubMed:10882114). Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (PubMed:15146077).

• Western blot detection of SF3B3 in SHSY-5Y,C6 cell lysates using SF3B3 Rabbit mAb(1:1000 diluted).Predicted band size:136KDa.Observed band size:136KDa.
• Immunocytochemistry of SF3B3(green) in Hela cells using SF3B3 Rabbit mAb at dilution 1/50, and DAPI(blue)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting