• Product NameTRIM56 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S02-8A3
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IF
• Species ReactivityHuman
• Immunogen DescriptionA synthetic peptide of human TRIM56
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    RING finger protein 109; RNF109
• Accession No. Swiss-Prot:Q9BRZ2
  GeneID:81844
• UniprotQ9BRZ2
• Gene ID81844
• Calculated MW Calculated MW: 82 kDa; Observed MW: 82 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/2000-1/10000; ICC/IF: 1/50;

Swiss-Prot Acc.Q9BRZ2.E3 ubiquitin-protein ligase that plays a key role in innate antiviral immunity (PubMed:21289118). In response to pathogen- and host-derived double-stranded DNA (dsDNA), targets TMEM173/STING to 'Lys-63'-linked ubiquitination, thereby promoting its homodimerization, a step required for the production of type I interferon IFN-beta . Independently of its E3 ubiquitin ligase activity, positive regulator of TLR3 signaling. Potentiates extracellular double stranded RNA (dsRNA)-induced expression of IFNB1 and interferon-stimulated genes ISG15, IFIT1/ISG56, CXCL10, OASL and CCL5/RANTES. Promotes establishment of an antiviral state by TLR3 ligand and TLR3-mediated chemokine induction following infection by hepatitis C virus (PubMed:22948160).

• Western blot detection of TRIM56 in Hela cell lysates using TRIM56 Rabbit mAb(1:1000 diluted).Predicted band size:82kDa.Observed band size:82kDa.
• Immunocytochemistry of TRIM56(green) in Hela cells using TRIM56 Rabbit mAb at dilution 1/50, and DAPI(blue)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting