• Product NameTRUB2 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S08-5D1
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB
• Species ReactivityHuman
• Immunogen DescriptionRecombinant protein of human TRUB2
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    Mitochondrial mRNA pseudouridine synthase TRUB2
• Accession No. Swiss-Prot:O95900
  GeneID:26995
• UniprotO95900
• Gene ID26995
• Calculated MW Calculated MW: 37 kDa; Observed MW: 37 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000

Swiss-Prot Acc.O95900.Minor enzyme contributing to the isomerization of uridine to pseudouridine (pseudouridylation) of specific mitochondrial mRNAs (mt-mRNAs) such as COXI and COXIII mt-mRNAs (PubMed:27974379). As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mitochondrial ribosomal RNA (16S mt-rRNA), controls 16S mt-rRNA abundance and is required for intra-mitochondrial translation (PubMed:27667664).

• Western blot detection of TRUB2 in K562,Hela cell lysates using TRUB2 Rabbit mAb(1:1000 diluted).Predicted band size:37kDa.Observed band size:37kDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting