• Product NameVEGFA Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S04-6D7
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human VEGFA
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    VEGFA; VEGF; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF
• Accession No. Swiss-Prot:P15692
  GeneID:7422
• UniprotP15692
• Gene ID7422
• Calculated MW Calculated MW: 27 kDa; Observed MW: 25-50 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000

Swiss-Prot Acc.P15692.Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development .

• Western blot detection of VEGFA in RAW264.7 cell lysates using VEGFA Rabbit mAb(1:1000 diluted).Predicted band size:27KDa.Observed band size:27KDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting