• Product NameXLF Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S08-9D7
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant protein of human XLF
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    Cernunno; Nhej1; Non homologous end joining factor 1; Protein cernunnos; XLF; XRCC4 like factor;
• Accession No. Swiss-Prot:Q9H9Q4
  GeneID:
• UniprotQ9H9Q4
• Calculated MW Calculated MW: 33 kDa; Observed MW: 39 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000;

Swiss-Prot Acc.Q9H9Q4.DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary (PubMed:16439204, PubMed:16439205, PubMed:17470781). Binds DNA in a length-dependent manner (PubMed:17317666).

• Western blot detection of XLF in K562,Hela cell lysates using XLF Rabbit mAb(1:1000 diluted).Predicted band size:33kDa.Observed band size:39kDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting