• Product NameSurfeit Locus Protein 4 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S07-1F1
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human SURF4
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    ERV29
• Accession No. Swiss-Prot:O15260
  GeneID:6836
• UniprotO15260
• Gene ID6836
• Calculated MW Calculated MW:30 kDa,Observed MW:30 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/2000 ICC/IF: 1/20

Endoplasmic reticulum cargo receptor that mediates the export of lipoproteins by recruiting cargos into COPII vesicles to facilitate their secretion (PubMed:30251625, PubMed:29643117, PubMed:33186557).Acts as a cargo receptor for lipoproteins bearing both APOB and APOA1, thereby regulating lipoprotein delivery and the maintenance of lipid homeostasis (PubMed:29643117, PubMed:33186557).Synergizes with the GTPase SAR1B to mediate transport of circulating lipoproteins (PubMed:33186557).Promotes the secretion of PCSK9 (PubMed:30251625).Also mediates the efficient secretion of erythropoietin (EPO) (PubMed:32989016).May also play a role in the maintenance of the architecture of the endoplasmic reticulum-Golgi intermediate compartment and of the Golgi (PubMed:18287528).

• Western blot detection of SURF4 in C6,Jurkat cell lysates using SURF4 Rabbit mAb(1:1000 diluted).Predicted band size:30kDa.Observed band size:30kDa.
• Immunocytochemistry of SURF4 (green) in Hela using SURF4 antibody at dilution 1/20, and DAPI(blue)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting