• Product NameCD147 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S04-5B3
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human CD147
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    OK; 5F7; TCSF; CD147; EMMPRIN
• Accession No. Swiss-Prot:P35613
  GeneID:682
• UniprotP35613
• Gene ID682
• Calculated MW Calculated MW:42 kDa,Observed MW:50 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000-1/5000 IHC: 1/50-1/200

Essential for normal retinal maturation and development (By similarity).Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed:25957687).In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed:25957687).May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes (PubMed:21620857).

• Western blot detection of CD147 in Hela,Ramos cell lysates using CD147 Rabbit mAb(1:1000 diluted).Predicted band size:42kDa.Observed band size:42kDa.
• Immunohistochemistry of CD147 in paraffin-embedded Human colon cancer tissue using CD147 Rabbit mAb at dilution 1/50

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting