• Product NameNEDD4 Rabbit mAb
• Clone No.S06-7C3
• IsotypeIgG
• PurificationAffinity-chromatography
• ApplicationsWB
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthesized peptide derived from human NEDD4
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    RPF1; NEDD4-1
• Accession No. Swiss-Prot:P46934
  GeneID:4734
• UniprotP46934
• Gene ID4734
• Calculated MW 149 kDa
• SDS-PAGE MW 135 kDa
• Concentration 0.6mg/ml
• Formulation Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB 1:1000-1:2000

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity.

• Western blot analysis of NEDD4 expression in A549 cell lysate.
• All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting