• Product NamePhospho-CDK1/2 (Thr14) Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S06-1A5
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic phosphopeptide corresponding to residues surrounding Thr14 of human Cdk1/2
• ConjugatesUnconjugated
• ModificationPhosphorylated
• Alternative Names    CDKN2; p33(CDK2)
• Accession No. Swiss-Prot:P06493 P24941
  GeneID:1017
• UniprotP06493 P24941
• Gene ID1017
• Calculated MW Calculated MW:34 kDa,Observed MW:34 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000; IHC: 1/50;

Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins (PubMed:16407259, PubMed:17459720, PubMed:16933150, PubMed:18356527, PubMed:19509060, PubMed:20171170, PubMed:19917720, PubMed:20937773, PubMed:20935635, PubMed:21063390, PubMed:23355470, PubMed:23601106, PubMed:23602554, PubMed:25556658, PubMed:26829474, PubMed:30704899).

• Western blot detection of Cdk1/2 (phospho-Thr14) in HepG2,U2OS,U251 using Cdk1/2 (phospho-Thr14) Rabbit mAb(1:1000 diluted)
• Immunohistochemistry of Cdk1/2 (phospho-Thr14) in paraffin-embedded Human lung cancer tissue using Cdk1/2 (phospho-Thr14) Rabbit mAb at dilution 1/50

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting