• Product NamePhospho-Filamin A/B (Ser2152/Ser2107) Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S02-7C0
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman
• Immunogen DescriptionA synthetic phosphopeptide corresponding to residues surrounding Ser2152 of human Filamin A
• ConjugatesUnconjugated
• ModificationPhosphorylated
• Alternative Names    FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FGS2; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280
• Accession No. Swiss-Prot:O75369
  GeneID:2316
• UniprotO75369
• Gene ID2316
• Calculated MW Calculated MW:281 kDa,Observed MW:281 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000-1/5000 IHC: 1/20

Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro.

• Western blot detection of Filamin A (phospho-Ser2152)/Filamin B (phospho-Ser2107) in Hela,A549,U2OS,C6 using Filamin A (phospho-Ser2152)/Filamin B (phospho-Ser2107) Rabbit mAb(1:1000 diluted)
• Immunohistochemistry of Filamin A (phospho-Ser2152)/Filamin B (phospho-Ser2107) in paraffin-embedded Human Cholangiocarcinoma using Filamin A (phospho-Ser2152)/Filamin B (phospho-Ser2107) Rabbit mAb at dilution 1/50

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting