• Product NamePhospho-Histone H3 (Ser10/Thr11) Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S08-6C4
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic phosphopeptide corresponding to residues surrounding Ser10/Thr11 of human Histone H3
• ConjugatesUnconjugated
• ModificationPhosphorylated
• Alternative Names    H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A
• Accession No. Swiss-Prot:P68431
  GeneID:8350
• UniprotP68431
• Gene ID8350
• Calculated MW Calculated MW:15 kDa,Observed MW:17 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000 IHC: 1/20

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

• Western blot detection of Histone H3 (phospho-Ser10/Thr11) in Hela,A549,HL-60,C6 using Histone H3 (phospho-Ser10/Thr11) Rabbit mAb(1:1000 diluted)
• Immunohistochemistry of Histone H3 (phospho-Ser10/Thr11) in paraffin-embedded Human lung cancer tissue using Histone H3 (phospho-Ser10/Thr11) Rabbit mAb at dilution 1/50

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting