• Product NameBMI1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S08-7A6
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB
• Species ReactivityHuman,Mouse
• Immunogen DescriptionRecombinant protein of human Bmi1
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    BMI1; PCGF4; RNF51
• Accession No. Swiss-Prot:P35226
  GeneID:100532731
• UniprotP35226
• Gene ID100532731
• Calculated MW Calculated MW:37 kDa,Observed MW:43 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:15386022, PubMed:16359901, PubMed:26151332, PubMed:16714294, PubMed:21772249, PubMed:25355358, PubMed:27827373). The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (PubMed:21772249, PubMed:25355358). In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (PubMed:15386022, PubMed:26151332, PubMed:21772249).

• Western blot detection of Bmi1 in C6,Rat Brain,Ramos using Bmi1 Rabbit mAb(1:1000 diluted)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting