• Product NameClathrin Heavy Chain Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S02-6C4
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human Clathrin heavy chain
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    Hc; CHC; CHC17; MRD56; CLH-17; CLTCL2
• Accession No. Swiss-Prot:Q00610
  GeneID:1213
• UniprotQ00610
• Gene ID1213
• Calculated MW Calculated MW:192 kDa,Observed MW:192 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/2000-1/10000 IHC: 1/20

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:16968737, PubMed:21297582). The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed:23532825). Plays a role in early autophagosome formation (PubMed:20639872).

• Western blot detection of Clathrin heavy chain in Hela,A549,HL-60,U251,U87-MG cell lysates using Clathrin heavy chain Rabbit mAb(1:1000 diluted).Predicted band size:192kDa.Observed band size:192kDa.
• Immunohistochemistry of Clathrin heavy chain in paraffin-embedded Human colon cancer tissue using Clathrin heavy chain Rabbit mAb at dilution 1/50

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting