• Product NameMyosin 10 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S05-6D8
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human non-muscle Myosin IIB/MYH10
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    NMMHCB; NMMHC-IIB
• Accession No. Swiss-Prot:P35580
  GeneID:4628
• UniprotP35580
• Gene ID4628
• Calculated MW Calculated MW:229 kDa,Observed MW:229 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000

Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the central part but not the margins of spreading cells), and lamellipodial extension; this function is mechanically antagonized by MYH9.

• Western blot detection of non-muscle Myosin IIB/MYH10 in A549,HL-60,U251,U87-MG cell lysates using non-muscle Myosin IIB/MYH10 Rabbit mAb(1:1000 diluted).Predicted band size:229kDa.Observed band size:229kDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting