• Product NameOptineurin Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S01-2C5
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman
• Immunogen DescriptionRecombinant protein of human Optineurin
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    NRP; FIP2; HIP7; HYPL; ALS12; GLC1E; TFIIIA-INTP
• Accession No. Swiss-Prot:Q96CV9
  GeneID:10133
• UniprotQ96CV9
• Gene ID10133
• Calculated MW Calculated MW:66 kDa,Observed MW:75 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000 IHC: 1/50

Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8 (PubMed:27534431). Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation (PubMed:27534431). Plays a role in the activation of innate immune response during viral infection. Mechanistically, recruits TBK1 at the Golgi apparatus, promoting its trans-phosphorylation after RLR or TLR3 stimulation (PubMed:27538435).

• Western blot detection of Optineurin in Hela,A549,HL-60,U2OS,U87-MG cell lysates using Optineurin Rabbit mAb(1:1000 diluted).Predicted band size:66kDa.Observed band size:75kDa.
• Immunohistochemistry of Optineurin in paraffin-embedded Human Cholangiocarcinoma using Optineurin Rabbit mAb at dilution 1/50

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting