• Product NamePBK Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S07-0B0
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant protein of human PBK/SPK
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    SPK; CT84; TOPK; HEL164; Nori-3
• Accession No. Swiss-Prot:Q96KB5
  GeneID:55872
• UniprotQ96KB5
• Gene ID55872
• Calculated MW Calculated MW:36 kDa,Observed MW:40 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000 IHC: 1/500

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.

• Western blot detection of PBK/SPK in Hela,A549,HL-60,U2OS cell lysates using PBK/SPK Rabbit mAb(1:1000 diluted).Predicted band size:36kDa.Observed band size:40kDa.
• Immunohistochemistry of PBK/SPK in paraffin-embedded Human lung cancer tissue using PBK/SPK Rabbit mAb at dilution 1/50

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting