• Product NameSPI1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S02-1D7
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB IF
• Species ReactivityMouse
• Immunogen DescriptionA synthetic peptide of mouse PU.1/Spi1
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    Dis1; PU.1; Dis-1; Sfpi1; Spi-1; Sfpi-1; Tcfpu1; Tfpu.1
• Accession No. Swiss-Prot:P17433
  GeneID:20375
• UniprotP17433
• Gene ID20375
• Calculated MW Calculated MW:31 kDa,Observed MW:31 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000 ICC/IF: 1/50

Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.

• Western blot detection of PU.1/Spi1 in CHO-K1,C6 cell lysates using PU.1/Spi1 Rabbit mAb(1:1000 diluted).Predicted band size:31kDa.Observed band size:31kDa.
• Immunocytochemistry of PU.1/Spi1 (green) in Raw264.7 using PU.1/Spi1 antibody at dilution 1/50, and DAPI(blue)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting