• Product NameSPAK Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S06-2B0
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman
• Immunogen DescriptionA synthetic peptide of human SPAK
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    DCHT; PASK; SPAK
• Accession No. Swiss-Prot:Q9UEW8
  GeneID:27347
• UniprotQ9UEW8
• Gene ID27347
• Calculated MW Calculated MW:60 kDa,Observed MW:65 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000 IHC: 1/20

May act as a mediator of stress-activated signals. Mediates the inhibition of SLC4A4, SLC26A6 as well as CFTR activities by the WNK scaffolds, probably through phosphorylation. Phosphorylates RELT.

• Western blot detection of SPAK in Hela,A549,HL-60,U2OS cell lysates using SPAK Rabbit mAb(1:1000 diluted).Predicted band size:60kDa.Observed band size:65kDa.
• Immunohistochemistry of SPAK in paraffin-embedded Human Cholangiocarcinoma using SPAK Rabbit mAb at dilution 1/50

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting