• Product NameUbiquitin Conjugation Factor E4 B Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S04-6C6
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human UBE4B
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    E4; UFD2; HDNB1; UBOX3; UFD2A
• Accession No. Swiss-Prot:O95155
  GeneID:10277
• UniprotO95155
• Gene ID10277
• Calculated MW Calculated MW:146 kDa,Observed MW:146 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000 ICC/IF: 1/20

Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases (By similarity). May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase (By similarity). May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation (PubMed:17369820).

• Western blot detection of UBE4B in Hela,A549,HL-60,U2OS,U897-MG cell lysates using UBE4B Rabbit mAb(1:1000 diluted).Predicted band size:146kDa.Observed band size:146kDa.
• Immunocytochemistry of UBE4B (green) in MCF-7 using UBE4B antibody at dilution 1/20, and DAPI(blue)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting