• Product NameAcetyl-Histone H3 (Lys14) Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S06-2F5
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB
• Species ReactivityHuman,Rat
• Immunogen DescriptionA synthesized peptide derived from human Histone H3 (acetyl K14)
• ConjugatesUnconjugated
• ModificationAcetylated
• Alternative Names    H3 histone family, member A; H3/A; H31; H3FA; H3FB; H3FC:; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; histone 1, H3a; histone cluster 1, H3a; Histone H3.1;
• Accession No. Swiss-Prot:P68431
  GeneID:
• UniprotP68431
• Calculated MW 15kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/500-1/2000

Swiss-Prot Acc.P68431.H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers.

• Western blot detection of Acetyl-Histone H3 (Lys14) in A549,HL-60 cell lysates using Acetyl-Histone H3 (Lys14) Rabbit mAb(1:1000 diluted).Observed band size:15kDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting