• Product NameSIRT1 Antibody
• Host SpeciesChicken
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB IF
• Species ReactivityHu Ms Rt
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a 19 amino acid peptide near the amino terminus of human SIRT1.
• Target NameSIRT1
• Alternative Names    Sirtuin, NAD-dependent deacetylase sirtuin-1, SIR2L1, hSIR2, hSIRT1
• Accession No. Swiss-Prot:Q96EB6
  Gene ID:23411
• UniprotQ96EB6
• Gene ID23411;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and have important functions in the regulation of metabolism, growth and differentiation, inflammation, cellular survival, as well as in senescence and lifespan extension. Sirtuins, including SIRT1-7, are human homologs of yeast Sir2p. Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) which regulate cellular metabolism, e.g. energy metabolism, and thereby are associated with aging and several age-related diseases. SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically via lysine residue.

• Western blot analysis of SIRT1 in mouse liver tissue lysate with SIRT1 antibody at 1 ug/mL.
• Immunofluorescence of SIRT1 in rat lung tissue with SIRT1 antibody at 20 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting