• Product NameeIF2a(phospho-Ser49) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of eIF2a only when phosphorylated at Serine 49.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 49 (L-L-S(p)-E-L) derived from Human eIF2a.
• Target NameeIF2a
• ModificationPhospho
• Alternative Names    Eukaryotic translation initiation factor 2 subunit alpha
• Accession No. Swiss-Prot: P05198
  NCBI Protein: NP_004085.1
• UniprotP05198
• Gene ID1965;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 38kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.
De Haro, C. et al. (1996) FASEB J. 10, 1378-1387.
Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128.
Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.

• Western blot analysis of extracts from Hela cells untreated or treated with starvation using eIF2a(phospho-Ser49) Antibody #11511.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2α (Phospho-Ser49) Antibody #11511 (left) or the same antibody preincubated with blocking peptide #51511 (right).
• Immunofluorescence staining of methanol-fixed Hela cells using eIF2a(phospho-Ser49) Antibody #11511.
• Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using eIF2α (Phospho-Ser49) Antibody #11511.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting