• Product NameRecombinant Tau(Phospho-Thr217) Rabbit mAb(G122)
• Host SpeciesRabbit
• ClonalityMonoclonal
• Clone No.G122
• PurificationAffinity purification
• ApplicationsWB,ELISA
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of Tau only when phosphorylated at threonione 217.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 217(L-P-T(p)-P-P) derived from Human Tau.
• Target NameTau
• ModificationPhospho
• Alternative Names    MAPT; MTBT1; PHF-tau;
• Accession No. Swiss-Prot#: P10636;
  NCBI Gene#: 4137;
  NCBI Protein#: NP_058519.3.
• UniprotP10636
• SDS-PAGE MW 50-80kd
• Concentration 1.0 mg/ml
• Formulation Liquid in Tris-Gly containing 50% glycerol and 0.02% sodium azide.
• Storage Store at -20°C. Avoid freeze/thaw cycles.

•  Western blotting: 1:500~1:1000

 Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. 

Goedert M., Proc. Natl. Acad. Sci. U.S.A. 85:4051-4055(1988).
Goedert M., EMBO J. 8:393-399(1989).
Lee G., Neuron 2:1615-1624(1989).

• Western blot analysis of extracts from 293 cells, transfected with Tau and treated with calf intestinal phosphatase (CIP) using Tau(Phospho-Thr217) Rabbit mAb.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting