• Product NameAurora A(phospho-Thr288) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of Aurora A only when phosphorylated at threonine 288.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 288 (R-T-T(p)-L-M) derived from Human Aurora A.
• Target NameAurora A
• ModificationPhospho
• Alternative Names    AIK; ARK1; AURA; BTAK; STK6
• Accession No. Swiss-Prot: O14965
  NCBI Protein: NP_003591.2
• UniprotO14965
• Gene ID6790;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 48kd
  Immunohistochemistry: 1:50~1:100

Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity By similarity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.

Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65.
Hauf, S. et al. (2003) J Cell Biol 161, 281-94.
Walter, A.O. et al. (2000) Oncogene 19, 4906-16.
Zhou, H. et al. (1998) Nat Genet 20, 189-93.

• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Aurora A(Phospho-Thr288) Antibody #11519(left) or the same antibody preincubated with blocking peptide(right).
• Western blot analysis of extracts from HT29 cells, treated with Hydroxyurea or calf intestinal phosphatase (CIP), using Aurora A (phospho-Thr288) Antibody #11519.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting