• Product NameChk1 (Phospho-Ser280) Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB
• Species ReactivityHuman
• SpecificityPhospho-Chk1 (S280) Antibody detects endogenous levels of total Phospho-Chk1 (S280)
• Immunogen Description A synthesized peptide derived from human Phospho-Chk1 (S280)
• Alternative Names    Checkpoint kinase 1; Chek1; Chk1; rad27;
• Accession No. O14757
• Calculated MW 54kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

•   WB 1:500~1:2000

  Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles.

• Western blot analysis of Phospho-Chk1 (S280) expression in 293T treated with Calyculin A cell lysate.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting