• Product NameNRIP1 Rabbit pAb
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB
• Species ReactivityHuman, Mouse
• Immunogen DescriptionRecombinant fusion protein of human NRIP1(NP_003480.2).
• Alternative Names    NRIP1;RIP140
• UniprotP48552
• Gene ID8204
• Target Species Human
• Calculated MW 126kDa
• SDS-PAGE MW 150KDa
• Storage Store at -20℃. Avoid freeze / thaw cycles.|Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

•  WB,1:500 - 1:2000

Nuclear receptor interacting protein 1 (NRIP1) is a nuclear protein that specifically interacts with the hormone-dependent activation domain AF2 of nuclear receptors. Also known as RIP140, this protein modulates transcriptional activity of the estrogen receptor. 

• Western blot analysis of extracts of Mouse brain, using NRIP1 Polyclonal Antibody at 1:1000 dilution.
  Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
  Lysates/proteins: 25ug per lane.
  Blocking buffer: 3% nonfat dry milk in TBST.
  Detection: ECL Basic Kit.
  Exposure time: 3min.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting