- Product NameRecombinant Human Interleukin-2 Cys125Ser
- Host SpeciesEscherichia coli
- Purification> 97 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x107 IU/mg.
- EndotoxinLess than 1.0 EU/ug of rHuIL-2 Cys125Ser as determined by LAL method.
- Calculated MW Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids.
- Target Sequence MPTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFSQSIIS TLT
- Formulation Lyophilized from a 0.2 mm filtered concentrated solution in 20 mM PB, pH3.5, with 5 % Trehalose.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
