• Product NameRecombinant Human Interleukin-3 Receptor alpha/CD123 Fc Chimera Protein, Insect Cells Derived
• Host SpeciesInsect Cell
• Purification> 95 % by SDS-PAGE analyses.
• Biological ActivityTesting in progress.
• EndotoxinLess than 0.01 EU/ug of rHuIL-3 Rα/CD123-Fc as determined by LAL method.
• Calculated MW Approximately 60.3 kDa on SDS-PAGE under reducing conditions, containing 529 amino acids.
• Target Sequence AGMGTKEDPN PPITNLRMKA KAQQLTWDLN RNVTDIECVK DADYSMPAVN NSYCQFGAIS LCEVTNYTVR VANPPFSTWI LFPENSGKPW AGAENLTCWI HDVDFLSCSW AVGPGAPADV QYDLYLNVAN RRQQYECLHY KTDAQGTRIG CRFDDISRLS SGSQSSHILV RGRSAAFGIP CTDKFVVFSQ IEILTPPNMT AKCNKTHSFM HWKMRSHFNR KFRYELQIQK RMQPVITEQV RDRTSFQLLN PGTYTVQIRA RERVYEFLSA WSTPQRFECD QEEGANTRAW RIEGRMDEPK SSDKTHTCPP CPAPEFEGAP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PTPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0, with 0.02 % Tween-20.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

Interleukin-3 receptor (IL-3 R) is a heterodimeric structure composed of a 70 kDa IL-3 R alpha subunit (IL-3 Rα or CD123) and a 120-140 kDa IL-3 R beta subunit (IL-3 Rβ or CD131). IL-3Rα is a glycoprotein member of the hematopoietic receptor superfamily. IL-3 Rα binds IL-3 with relatively low affinity. In the presence of IL-3 Rβ, however, IL-3 R alpha has a much higher affinity for IL-3.Emerging studies demonstrate that CD123, the IL-3 Rα, is highly expressed in leukemic stem cells (LSCs), while not normal hematopoietic stem cells (HSCs), and associates with treatment response, minimal residual disease (MRD) detection and prognosis. Furthermore, CD123 is an important marker for the identification and targeting of LSCs for refractory or relapsed leukemia.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting