• Product NameRecombinant Human Interleukin-7
• Purification> 97% by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. rHuIL-7 stimulates proliferation of PHA-activated human peripheral blood mononuclear cell (PBMC). The specific activity of Recombinant Human IL-7 is > 1.0 x10⁸ Units/mg, which is calibrated against human IL-7 WHO Standard (NIBSC code: 90/530).
• EndotoxinLess than 1 EU/ug of rHuIL-7 as determined by LAL method.
• Alternative Names    LP-1, pre-B cell factor
• UniprotP13232
• Gene ID3574
• Calculated MW Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 150 mM NaCl, pH 7.4.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- A minimum of 12 months from date of receipt, when stored at ≤-20 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

Interleukin-7 (IL-7) is encoded by the IL7 gene and secreted by stromal cells in the red marrow and thymus. It binds to the IL-7 receptor, a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and also stimulates proliferation of B cells, T cells and NK cells. Murine IL-7 has approximately 65% amino acid sequence identity with human IL-7 and both proteins exhibit cross-species activity. IL-7 as an immunotherapy agent has been examined in many human clinical trials for various malignancies and during HIV infection.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting