• Product NameRecombinant Human Interleukin-16, 130 a.a.
• Host SpeciesEscherichia coli
• Purification> 95 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral T Lymphocytes is in a concentration range of 50-500 ng/ml.
• EndotoxinLess than 1 EU/ug of rHuIL-16, 130 a.a. as determined by LAL method.
• Alternative Names    Interleukin-16 isoform 2, Lymphocyte chemoattractant factor, LCF
• UniprotQ14005
• Gene ID3603
• Calculated MW Approximately 13.4 kDa, a single non-glycosylated polypeptide chain containing 130amino acids.
• Target Sequence MPDLNSSTDS AASASAASDV SVESTAEATV CTVTLEKMSA GLGFSLEGGK GSLHGDKPLT INRIFKGAAS EQSETVQPGD EILQLGGTAM QGLTRFEAWN IIKALPDGPV TIVIRRKSLQ SKETTAAGDS
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

Human Interleukin-16 (IL-16) is also called lymphocyte chemoattractant factor (LCF) and it has three isoforms which are cleaved from pre-Interleukin-16. IL-16 is mostly secreted by lymphocytes, epithelial cells, eosinophils, and CD8+ T cells. It has many functions including induction of the IL-2Rα on T cells, suppression of human immunodeficiency virus (HIV) replication, inhibition of T-cell antigen receptor/CD3 mediated T-cell stimulation in mixed lymphocyte reactions and so on. It signals through CD4 receptor. Furthermore, recombinant human IL-16 (130 a.a.) contains 130 amino acid residues and it shares 85 % a.a. sequence identity with murine IL-16.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting