- Product NameRecombinant Human Tumor Necrosis Factor-beta/TNFSF1
- Host SpeciesEscherichia coli.
- Purification> 96 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 5 pg/ml, corresponding to a specific activity of > 2.0 x108 IU/mg in the presence of actinomycin D.
- EndotoxinLess than 0.1 EU/ug of rHuTNF-β/TNFSF1 as determined by LAL method.
- Calculated MW Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 171 amino acids.
- Target Sequence LPGVGLTPSA AQTARQHPKM HLAHSTLKPA AHLIGDPSKQ NSLLWRANTD RAFLQDGFSL SNNSLLVPTS GIYFVYSQVV FSGKAYSPKA TSSPLYLAHE VQLFSSQYPF HVPLLSSQKM VYPGLQEPWL HSMYHGAAFQ LTQGDQLSTH TDGIPHLVLS PSTVFFGAFA L
- Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4, with 0.02 % Tween-20.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
