- Product NameRecombinant Human soluble Receptor Activator of Nuclear Factor kappa-B Receptor/TNFRSF11A
- Host SpeciesEscherichia coli.
- Purification> 98 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by its ability to inhibit sRANK Ligand induced nuclear factor kappa B(NFkappaB) in RAW 264.7 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 x104 IU/mg in the presence of 15 ng/ml of recombinant sRANK Ligand.
- EndotoxinLess than 0.1 EU/ug of rHusRANK Receptor as determined by LAL method.
- UniprotQ9Y6Q6
- Gene ID8792
- Calculated MW Approximately 19.1 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.
- Target Sequence QIAPPCTSEK HYEHLGRCCN KCEPGKYMSS KCTTTSDSVC LPCGPDEYLD SWNEEDKCLL HKVCDTGKAL VAVVAGNSTT PRRCACTAGY HWSQDCECCR RNTECAPGLG AQHPLQLNKD TVCKPCLAGY FSDAFSSTDK CRPWTNCTFL GKRVEHHGTE KSDAVCSSSL PARK
- Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150mM NaCl.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
