- Product NameRecombinant Human soluble Tumor Necrosis Factor Receptor Type II/TNFRSF1B
- Host SpeciesEscherichia coli.
- Purification> 97 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 cells is less than 0.2 μg/ml, corresponding to a specific activity of > 5000 IU/mg in the presence of 0.25 ng/mL of rHuTNF-α.
- EndotoxinLess than 0.1 EU/ug of rHusTNF RII/TNFRSF1B as determined by LAL method.
- UniprotP20333
- Gene ID7133
- Calculated MW Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 184 amino acids.
- Target Sequence MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPT
- Formulation Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
