- Product NameRecombinant Human Fibroblast Growth Factor 4
- Host SpeciesEscherichia coli.
- Purification> 96 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x106 IU/mg.
- EndotoxinLess than 1 EU/ug of rHuFGF-4 as determined by LAL method.
- Alternative Names HST, HST-1, HSTF-1, HBGF-4, Transforming Protein KS3
- UniprotP08620
- Gene ID2249
- Calculated MW Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids.
- Target Sequence GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL
- Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 1 x PBS, with 350 mM NaCl, 5% trehalose and 0.02% Tween-20, pH 7.0.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ˚C as supplied. - 1 month, 2 to 8 ˚C under sterile conditions after reconstitution. - 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
