• Product NameRecombinant Human Fibroblast Growth Factor 21
• Host SpeciesEscherichia coli.
• Purification> 96 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 x10³ IU/mg in the presence of 5 μg/ml of rMuKlotho-β and 10 μg/ml of heparin.
• EndotoxinLess than 1 EU/ug of rHuFGF-21 as determined by LAL method.
• UniprotQ9NSA1
• Gene ID26291
• Calculated MW Approximately 19.4 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids.
• Target Sequence HPIPDSSPLL QFGGQVRQRY LYTDDAQQTE AHLEIREDGT VGGAADQSPE SLLQLKALKP GVIQILGVKT SRFLCQRPDG ALYGSLHFDP EACSFRELLL EDGYNVYQSE AHGLPLHLPG NKSPHRDPAP RGPARFLPLP GLPPALPEPP GILAPQPPDV GSSDPLSMVG PSQGRSPSYA S
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

Fibroblast growth factor-21 (FGF-21) belongs to the large FGF family which is encoded by the FGF-21 gene and it is specifically induced by HMGCS2 activity. All FGF family members are heparin binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure and they are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. FGF-21 contains a 28 a.a. signal sequence and a 181 a.a. mature region but show limited binding to heparin. In addition, Mature human FGF-21 respectively shows 81 % a.a. identity to murine and rat FGF-21, and is known to be active on murine cells.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting