- Product NameRecombinant Human Platelet-derived Growth Factor-CC
- Host SpeciesEscherichia coli
- Purification> 97 % by SDS-PAGE analyses.
- Biological ActivityMeasured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is 70-350 ng/ml.
- EndotoxinLess than 0.1 EU/ug of rHuPDGF-CC as determined by LAL method.
- Calculated MW Approximately 27 kDa, a disulfide-linked homodimer of two 117 amino acid, C-terminal polyhistidine tagged proteins.
- Target Sequence Val235-Gly345, with an N-terminal Met and 6-His tag; Accession # NP_057289
- Formulation Lyophilized from 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ˚C as supplied. - 1 month, 2 to 8 ˚C under sterile conditions after reconstitution. - 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
