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Recombinant Human Transforming Growth Factor - beta 1 #AP60091

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Catalog No. Package Size Price Quantity
5ug $109.00
100ug $799.00
Availability:3-5days
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Description
  • Product NameRecombinant Human Transforming Growth Factor - beta 1
  • Host SpeciesChinese Hamster Ovary cell line, CHO
  • Purification> 97 % by SDS-PAGE analyses.
  • Biological ActivityMeasured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. The ED50 for this effect is 0.04-0.2 ng/ml. The specific activity of rHuTGF-β1 is approximately 2.5 x104 U/μg, which is calibrated against human TGF-β1 Standard.
  • EndotoxinLess than 0.1 EU/ug of rHuTGF-β1 as determined by LAL method.
  • Calculated MW Apparent molecular mass of 24 kDa in SDS-PAGE under non-reducing conditions, 12 kDa under reducing conditions, a disulfide-linked homodimer of two 112 amino acid glycosylated polypeptide chains.
  • Target Sequence Ala279-Ser390; Accession # P01137
  • Formulation Lyophilized from 0.2 μm filtered concentrated solution in 35 % Acetonitrile and 0.1 % TFA.
  • Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
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Application

WBWestern Blotting

IHCImmunohistochemistry

IFImmunofluorescence

ICCImmunocytochemistry

FCFlow Cytometry

IPImmunoprecipitation

EELISA

DBDot Blotting

ChIPChromatin Immunoprecipitation

GICAGold Immunochromatography Assay

NCNegative Control

Species Reactivity

HuHuman

MsMouse 

RtRat 

Dm Drosophila melanogaster

C Caenorhabditis elegans

MkMonkey

RbRabbit

B Bovine 

D Dog

PPig

HmHamster

ChHm Chinese Hamster 

ChkChicken  

ShpSheep