• Product NameRecombinant Human Ciliary Neurotrophic Factor
• Host SpeciesE.coli
• Purification> 97 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 �� 10³ IU/mg.
• EndotoxinLess than 1 EU/ug of rHuCNTF as determined by LAL method.
• Alternative Names    CNTF
• UniprotP26441
• Gene ID1270
• Calculated MW Approximately 22.9 kDa, a single non-glycosylated polypeptide chain containing 200 amino acids.
• Target Sequence MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM
• Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ��C as supplied.- 1 month, 2 to 8 ��C under sterile conditions after reconstitution.- 3 months, -20 to -70 ��C under sterile conditions after reconstitution.

Ciliary neurotrophic factor (CNTF) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. It was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. The protein is also a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. In addition, CNTF is useful for treatment of motor neuron disease and it could reduce food intake without causing hunger or stress. CNTF is structurally related to IL-6, IL-11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes. Recombinant human CNTF containing 200 amino acids and it shares 82 % and 83 % a.a. sequence identity with mouse and rat CNTF.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting