• Product NameRecombinant Human/Murine/Rat Activin A
• Host SpeciesMammalian Cell
• Purification> 95 % by SDS-PAGE analyses.
• Biological ActivityTesting in progress.
• EndotoxinLess than 0.01 EU/ug of rHu/Mu/RtActivin A as determined by LAL method.
• UniprotP08476
• Calculated MW Apparent molecular mass of 24 kDa in SDS-PAGE under non-reducing conditions, 14 kDa under reducing conditions, a disulfide-linked homodimer of two 116 amino acid glycosylated polypeptide chains.
• Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ��C as supplied.- 1 month, 2 to 8 ��C under sterile conditions after reconstitution.- 3 months, -20 to -70 ��C under sterile conditions after reconstitution.

Activins and Inhibins are TGF-β superfamily cytokines that are involved in tissue morphogenesis and repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive system function, and carcinogenesis. Activin A protects the heart from hypoxic stress and promotes the differentiation of embryonic stem cells into cardiomyocytes. Activins are homodimers or heterodimers of various β subunits (βA, βB, βC, and βE), while Inhibins are heterodimers of a unique α subunit and one of the β subunits. Activin A is a homodimer of two βA chains. Human βA shares 100% amino acid sequence identity with mouse, rat, bovine, porcine, and feline βA. Activin A binds to Activin RIIA which then associates with Activin RIB/ALK-4. Activin A bioactivity is regulated by cell-associated molecules (BAMBI, Betaglycan, and Cripto) and soluble molecules (β2-Macroglobulin, Follistatin, and FLRG).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting