• Product NameRecombinant Rhesus Macaque Interleukin-4
• Host SpeciesEscherichia coli.
• Purification> 96 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 x10⁶ IU/mg.
• EndotoxinLess than 1 EU/ug of rRhIL-4 as determined by LAL method.
• Alternative Names    BSF-1, Lymphocyte Stimulatory Factor 1
• UniprotP51492
• Calculated MW Approximately 14.9 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.
• Target Sequence HNCHIALREI IETLNSLTEQ KTLCTKLTIT DILAASKNTT EKETFCRAAT VLRQFYSHHE KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE DFLERLKTIM REKYSKCSS
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 2 x PBS, pH 7.4, 5 % trehalose.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

Interleukin-4 (IL-4) is a pleiotropic cytokine mainly produced by mast cells, activated T cells and bone marrow stromal cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. Additionally, It enhances both secretion and cell surface expression of IgE and IgG1 and also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. The rhesus macaque IL-4 shares about 40 % ~ 45 % a.a. sequence identity with human, mouse, rat IL-4 and they are species-specific in their activities.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting