• Product NameRecombinant Rhesus Macaque Interleukin-16
• Host SpeciesEscherichia coli.
• Purification> 98 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral T lymphocytes is in a concentration range of 1.0-100 ng/ml.
• EndotoxinLess than 1 EU/ug of rRhIL-16 as determined by LAL method.
• Alternative Names    Interleukin-16, Lymphocyte Chemoattractant Factor, LCF
• UniprotO62675
• Gene ID574100
• Calculated MW Approximately 12.5 kDa, a single non-glycosylated polypeptide chain containing 121 amino acids.
• Target Sequence SAASASAASD VSVESSAEAT VYTVTLEKMS AGLGFSLEGG KGSLHGDKPL TINRIFKGAA SEQSETIQPG DEILQLAGTA MQGLTRFEAW NIIKALPDGP VTIVIRRKSL QPKETTAAAD S
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

Interleukin-16 (IL-16) is also called lymphocyte chemoattractant factor (LCF) and it is mostly secreted by lymphocytes, epithelial cells, eosinophils, and CD8+ T cells. It has many functions including induction of the IL-2Rα on T cells, suppression of human immunodeficiency virus (HIV) replication, inhibition of T-cell antigen receptor/CD3 mediated T-cell stimulation in mixed lymphocyte reactions and so on. It signals through CD4 receptor. Furthermore, recombinant rhesus macaque interleukin-16 contains 121 amino acid residues and it shares 85 % ~ 95 % a.a. sequence identity with human and murine IL-16.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting