- Product NameRecombinant Murine Interleukin-21
- Host SpeciesEscherichia coli.
- Purification> 98 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human N1186 T cells is less than 25 ng/ml, corresponding to a specific activity of > 4.0 x104 IU/mg.
- EndotoxinLess than 0.1 EU/ug of rMuIL-21 as determined by LAL method.
- UniprotQ9ES17
- Gene ID60505
- Calculated MW Approximately 15.0 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.
- Target Sequence HKSSPQGPDR LLIRLRHLID IVEQLKIYEN DLDPELLSAP QDVKGHCEHA AFACFQKAKL KPSNPGNNKT FIIDLVAQLR RRLPARRGGK KQKHIAKCPS CDSYEKRTPK EFLERLKWLL QKMIHQHLS
- Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 2 x PBS, pH 7.4.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
