- Product NameRecombinant Murine Thrombopoietin
- Host SpeciesEscherichia coli.
- Purification> 95 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human MO7e cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 x106 IU/mg.
- EndotoxinLess than 0.1 EU/ug of rMuTPO as determined by LAL method.
- UniprotP40226
- Gene ID21832
- Calculated MW Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.
- Target Sequence SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF
- Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5% Trehalose.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
