- Product NameRecombinant Murine TNF-related apoptosis-inducing Ligand/TNFSF10
- Host SpeciesEscherichia coli.
- Purification> 95 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x106 IU/mg in the presence of actinomycin D.
- EndotoxinLess than 0.1 EU/ug of rMuTRAIL/TNFSF10 as determined by LAL method.
- UniprotP50592
- Gene ID22035
- Calculated MW Approximately 20.2 kDa, a single non-glycosylated polypeptide chain containing 175 amino acids.
- Target Sequence MPRGGRPQKV AAHITGITRR SNSALIPISK DGKTLGQKIE SWESSRKGHS FLNHVLFRNG ELVIEQEGLY YIYSQTYFRF QEAEDASKMV SKDKVRTKQL VQYIYKYTSY PDPIVLMKSA RNSCWSRDAE YGLYSIYQGG LFELKKNDRI FVSVTNEHLM DLDQEASFFG AFLIN
- Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 3 mM DTT.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
