- Product NameRecombinant Murine LIGHT/TNFSF14
- Host SpeciesEscherichia coli.
- Purification> 96 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using human HT-29 cells is less than 2 μg/ml, corresponding to a specific activity of > 500 IU/mg in the presence of murine anti-polyHistidine monoclonal antibody and rHuIFN-γ.
- EndotoxinLess than 1 EU/ug of rMuLIGHT/TNFSF14 as determined by LAL method.
- Calculated MW Approximately 18.4 kDa, a single non-glycosylated polypeptide chain containing 168 amino acids.
- Target Sequence DGGKGSWEKL IQDQRSHQAN PAAHLTGANA SLIGIGGPLL WETRLGLAFL RGLTYHDGAL VTMEPGYYYV YSKVQLSGVG CPQGLANGLP ITHGLYKRTS RYPKELELLV SRRSPCGRAN SSRVWWDSSF LGGVVHLEAG EEVVVRVPGN RLVRPRDGTR SYFGAFMV
- Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
