- Product NameRecombinant Murine Vascular Endothelial Growth Factor 120, Yeast
- Host SpeciesYeast
- Purification> 95 % by SDS-PAGE and 90% by SEC-HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 1-4 ng/ml.
- EndotoxinLess than 0.1 EU/ug of rMuVEGF120, Yeast as determined by LAL method.
- Calculated MW Theoretically as a disulfide-linked homodimeric protein, the product consists of two 121 amino acid polypeptide chains. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from 20.7 kDa in SDS-PAGE under reducing conditions.
- Target Sequence MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R
- Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 ˚C as supplied.1 month, 2 to 8 ˚C under sterile conditions after reconstitution.3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
