- Product NameRecombinant Murine Vascular Endothelial Growth Factor 164, Yeast
- Host SpeciesYeast
- Purification> 97 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is between 1.0-5.0 ng/ml.
- EndotoxinLess than 0.01 EU/ug of rMuVEGF164, Yeast as determined by LAL method.
- Calculated MW Theoretically as a disulfide-linked homodimeric protein, the product consists of two 165 amino acid polypeptide chains. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from approximately 40 kDa in SDS-PAGE under non-reducing conditions.
- Target Sequence MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
- Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 ˚C as supplied.1 month, 2 to 8 ˚C under sterile conditions after reconstitution.3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
